The European Commission regulation on maximum levels for certain contaminations in food describes maximum tolerated levels of four per- and polyfluoroalkyl substance (PFAS) compounds in various food matrices such as meat and fish products, as well as eggs. Additionally, there is a European Commission recommendation to monitor additional PFAS components. Other regions around the world are working on similar regulations. This application note presents the development and validation of a multicomponent method for the analysis of 21 PFAS compounds in chicken eggs. The method incorporates a sample extraction using QuEChERS cleanup, followed by solid-phase extraction using Agilent Bond Elut Carbon S cartridges. Quantitative analysis was performed by LC/MS/MS using the Agilent 1290 Infinity II LC system coupled to the Agilent 6475A triple quadrupole LC/MS. The method was validated according to SANTE guidance 11312/2021, monitoring analyte extraction recoveries, linearity, sensitivity (method detection limits), and reproducibility.