This application note presents the development and validation of a multiresidue method for the analysis of per- and polyfluoroalkyl substances (PFAS) in infant formula, milk, and eggs. The method uses QuEChERS extraction followed by EMR mixed-mode passthrough cleanup using an Agilent Captiva EMR PFAS Food II cartridge, then LC/MS/MS detection. The method features a simplified and efficient sample preparation, sensitive LC/MS/MS detection, and reliable quantitation using neat standard calibration curves. The novel Captiva EMR PFAS Food II cartridge was developed and optimized specifically for PFAS analysis in foods of animal origin as well as seeded dry foods of plant origin. The method was validated based on the AOAC Standard Method Performance Requirements (SMPR) 2023.003, including method suitability, sensitivity, accuracy, and precision. The method was demonstrated to meet the required limits of quantitation (LOQs), recovery, and repeatability (RSD) for four core PFAS targets—perfluorooctane sulfonic acid (PFOS), perfluorooctanoic acid (PFOA), perfluoronanoic acid (PFNA), and perfluorohexane sulfonic acid (PFHxS)—and 26 remaining PFAS targets in infant formula, milk, and eggs.